A team of researchers at the Southern Medical University, Guangzhou, China, and collaborators report development of a rapid diagnostic assay for detection of SARS-CoV-2 antibodies in human blood. They report accurate detection with patient samples, with only 10 minutes to get a readout per blood sample.
The new method is a lateral flow assay, much like a home pregnancy test. Human serum is obtained from a test patient and added onto one side of the device, where it flows through the paper due to capillary action. The researchers impregnated COVID-19 proteins in a thin line on this paper to bind the antibodies. If the anti-COVID-19 antibodies are present, they will bind along this line, else, there will be no binding activity. To improve resolution, the researchers also use secondary binding of rabbit anti-human IgG antibodies, linked with a fluorescent reporter, allowing for greater accuracy than an unassisted visual readout.